[Effect of low - level helium-neon laser irradiation on the release of interleukin 6 and basic fibroblast growth factor from cultured human fibroblasts in high glucose medium]

Low level laser therapy is suggested as a new therapeutic method in diabetic wound healing. This survey aimed to evaluate the effects of low level laser on human fibroblasts cultured in high glucose cultures. The human skin fibroblasts were cultured under standard condition. The cells were cultured in high glucose culture medium [15mM/L] for a week and two weeks before laser irradiation. The irradiation was carried out with Helium-Neon [He-Ne] laser unit on the case groups. Control cells did not receive laser. The cells were irradiated with three doses of 0.5, 1 and 2 J/cm2 on three consecutive days. The releasing rates of Interleukin 6 and Fibroblast growth factor were evaluated by ELISA [enzyme-linked immunoabsorbent assay]. Data were analyzed by student t test or Mann-Whitney-U test. All three doses of 0.5 [p=0.049], 1 J/cm2 [p=0.027] had a stimulatory effect on releasing rate of IL-6 of the fibroblasts cultured in culture medium with high glucose concentration in comparison with hyper glycemic non irradiated cells. Low level laser therapy [LLLT] with energy density of 2 J/cm2 induced the release of bFGF of the fibroblasts cultured in culture medium with high glucose concentration for one and two weeks [p=0.047 and p=0.04 respectively]. This study showed that LLLT has stimulatory effects on the release of Interleukin 6 and basic fibroblast growth factor from cultured human fibroblasts in high glucose medium in one week and two-week tests

[Effects of different growth factors on new bone formation: a systematic review]

Using different osteogenic growth factors, which is still under investigation, is a favorable method for bone regeneration. This systematic review is aimed at evaluating the effects of different growth factors and their carriers on osteogenesis. Electronic databases [Medline, EMBase and Cochrane] were searched by the authors for articles published from 1999 to April 2010. Clinical and animal studies evaluating bone formation by applying a specific growth factor and the related carrier were included in this investigation. Obtained data were organized in a table and evaluated through a qualitative analysis. Sixty-three studies most of which evaluated the effects of BMP-2 osteogenesis in different models were included in this study. Totally, twenty-five carriers were applied with different growth factors in the experiments. Among these carriers, poly lactic- co glycolic acid [PLGA], hydroxy apatite/tricalcium phosphate/absorbable collagen sponge [HA/TCP/ACS] and BioOss were the most frequently used carriers with the growth factors in bone regeneration studies. The current evidence, although not strong enough, confirms that BMP-2 has more favorable results in osteogenesis compared with other factors. The carrier scaffold, methods of measurement [histologic or radiographic], type of animal, defect diameter and the length of follow-up are the variables that should be matched before reaching definite results for the effect of growth factors in bone regeneration

[Effect of low - level helium - neon laser irradiation on proliferation rate of cultured human fibroblasts in high glucose medium]

This study aimed to evaluate the effects of low level laser on human fibroblasts which were cultured in high glucose cultures. The human skin fibroblasts were maintained in Dulbecco's Modified Eagle's Medium [DMEM] in a humidified air-5% CO[2] atmosphere. The cells were cultured. Irradiation was carried out with Helium-Neon [He-Ne] laser unit with power output of 1.5 mW and the diameter of irradiating beam of 1.7 cm on the case groups. In the next step the cells were cultured in high glucose culture medium [15mM/L] for one week before laser irradiation. The irradiation was carried out with the same laser unit with power output of 1.5 mW and the diameter of irradiating beam of 1.7 cm on the case groups. Control cells did not receive laser. The cells were irradiated with three doses of 0.5, 1 and 2 J/cm[2] on three consecutive days. Cell proliferation was evaluated by dimethylthiazol-diphenyltetrazolium bromide [MTT] assay. Data were analyzed by student t test or Mann-Whitney-U test. Laser treatments of 0.5 [p-value=0.002] and 1 [p-value=0.046] had a stimulatory effect and three irradiations of 2 J/cm[2] had a negative effect [p-value=0.011] on proliferation rate of fibroblasts which were cultured in normal culture medium compared to non irradiated cells. All three doses of 0.5 [p-value=0.042], 1 [p-value=0.00] and 2 J/cm[2] [p-value=0.00] had a stimulatory effect on proliferation rate of fibroblasts which were cultured in culture medium with high glucose concentration [15mM/L] in comparison with hyper glycemic non irradiated cells. Low level laser irradiation with helium-neon unit on cultured human fibroblasts in normal and high glucose mediums increased their growth and proliferation

[Appearance of anatomical structures of mandible on panoramic radiographs [panorex X-ray] in patients who referred to dental school of Shahid Beheshti University]

Currently panoramic radiography is used for diagnostic dental, and bone lesions, but anatomical structures also can see, for example incisive canal.At present time incisive canal position is investigated in Iranian population. Panoramic radiographs were taken by Planmeca machine from 412 patients using standard exposure. The position of patient was in compliance with standard protocol. After that mandibular incisive canal was evaluated, and data were analyzed statistically. Mental foramen, anterior loop of mental nerve and incisive canal could be observed in 84.2, 66 and 51.7% of the cases. Respectively lingual foramen wich was observed in only 6.1% of the radiographs.Gender doesn't affect on the visibility of these structures.There was a relationship between mandibular foramen and canal with age among those six landmarks. The most percent of visibility of all the landmarks was in young group and adults [third and fourth decade]. Mandibular incisive canal was considered in 51.7% of cases, it was obsereved thatthe result obtained was more than those of other researches